Detection of Bacteroides fragilis Enterotoxin Gene by PCR
نویسندگان
چکیده
منابع مشابه
Detection of Bacteroides fragilis enterotoxin gene by PCR.
Bacteroides fragilis constitutes about 1% of the bacterial flora in intestines of normal humans. Enterotoxigenic strains of B. fragilis have been associated with diarrheal diseases in humans and animals. The enterotoxin produced by these isolates induces fluid changes in ligated intestinal loops and an in vitro cytotoxic response in HT-29 cells. We developed a nested PCR to detect the enterotox...
متن کاملDetection of enterotoxigenic Bacteroides fragilis by PCR.
Strains of enterotoxigenic Bacteroides fragilis (ETBF) are associated with diarrhea in young farm animals and, at least in particular settings, in children. Enterotoxin production by ETBF is currently detected by a tissue culture assay with HT-29 cells. We have developed a PCR assay based on the detection of the enterotoxin gene to identify ETBF in culture and in stool samples. Overall, 113 bac...
متن کاملPCR Detection of the Bacteroides fragilis Enterotoxin Gene Relies on Robust Primer Design.
Colonic carriage of enterotoxigenic Bacteroides fragilis (ETBF) is reportedly more common in people presenting with colorectal cancer (CRC) than in healthy controls (1–3), fueling speculation that persistent carriage of these bacteria may “drive” colon carcinogenesis (4). Our aim was to use a bft amplification protocol to determine the prevalence of toxin-producing strains of B. fragilis in CRC...
متن کاملBacteroides fragilis enterotoxin cleaves the zonula adherens protein, E-cadherin.
Strains of Bacteroides fragilis associated with diarrheal disease (enterotoxigenic B. fragilis) produce a 20-kDa zinc-dependent metalloprotease toxin (B. fragilis enterotoxin; BFT) that reversibly stimulates chloride secretion and alters tight junctional function in polarized intestinal epithelial cells. BFT alters cellular morphology and physiology most potently and rapidly when placed on the ...
متن کاملOccurrence of Bacteroides fragilis enterotoxin gene-carrying strains in Germany and the United States.
Ninety-three Bacteroides fragilis isolates from different geographic locations were analyzed for the presence of an enterotoxin-encoding gene. It was shown that blood culture isolates were more likely to carry this gene than strains from other sources. All enterotoxin-positive strains belonged to the PCR fingerprint group I.
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1998
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.36.6.1729-1732.1998